Direct PCR detection of Escherichia coli O157:H7
نویسندگان
چکیده
منابع مشابه
Direct PCR detection of Escherichia coli O157:H7.
AIMS This paper reports a simple, rapid approach for the detection of Shiga toxin (Stx)-producing Escherichia coli (STEC). METHODS AND RESULTS Direct PCR (DPCR) obviates the need for the recovery of cells from the sample or DNA extraction prior to PCR. Primers specific for Stx-encoding genes stx1 and stx2 were used in DPCR for the detection of E. coli O157:H7 added to environmental water samp...
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Escheric hia coli O157:H7 lives in the intestines of healthy cattle, and can contaminate meat during slaughtering pr actices . Detection of the low infectious dosage of bacterium requires a sensitive method. We developed polymerase chain reaction (PCR) assays to detect the gene Stx2 irrespective of the bacterial serotype. In this study, the detection limit of the PCR protocol in detecting Stx...
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2003
ISSN: 0266-8254,1365-2673
DOI: 10.1046/j.1472-765x.2003.01386.x